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KMID : 1145120170150040523
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 4 p.523 ~ p.530
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Anti-oxidant Effects of Scutellarin in UVA-irradiated HaCaT Cells
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Jeon See-Un
Kim Soo-Yeon
Lee Na-Kyeong
Bae Seung-Hee
Lee Jae-Nam
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Abstract
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Purpose: This study was to investigate the anti-oxidant activity of scutellarin in ultraviolet A (UVA)-irradiated HaCaT cells.
Methods: To confirm the anti-oxidant effects of scutellarin, water-soluble tetrazolium salt (WST-1) was used to determine the cytotoxicity, 2′,7′-dichlorofluorescin diacetate (DCFDA) was used to quantitatively analyze intracellular reactive oxygen species (ROS), and quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the regulation of superoxide dismutase 1 (SOD1), catalase (CAT) and glutathione peroxidase 1 (GPx1) mRNA expression.
Results: As a result, scutellarin had no toxicity, and effectively protected HaCaT cells from UVA in a concentration dependent manner. The intracellular ROS was increased by 2.15 times after cell irradiated with UVA, but decreased by 1.61-1.20 times in scutellarin pretreatment group. The expression of SOD1, CAT and GPx1 mRNA, which was downgraded by UVA irradiation, was upregulated in a concentration dependent manner in the scutellarin pretreatment group.
Conclusion: This study confirmed that scutellarin regulated anti-oxidant defense mechanisms in HaCaT cells. Future research will be needed to confirm the application of scutellarin as a material for the development of functional cosmetics.
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KEYWORD
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Scutellarin, Human keratinocyte, Ultraviolet A, Anti-oxidant, Reactive oxygen species
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